12 Methylene Blue Benefits

The 12 Benefits of Methylene Blue are listed below.

• Boosts Energy and Reduces Fatigue: Methylene blue boosts mitochondrial function, supports neuroprotection, and reduces oxidative stress, contributing to sustained neuronal health, improved cognition, and the mitigation of neurodegenerative diseases.

• Enhances Cognitive Function and Mental Clarity: Methylene blue increases mitochondrial efficiency, reduces oxidative stress, stabilizes ATP production, and minimizes reactive oxygen species. 

• Protects Brain Health and Prevents Degeneration: Methylene blue benefits brain health and prevents degeneration by improving mitochondrial function, increasing ATP production, and reducing oxidative stress in neurons.

• Improved Skin Health and Slows Aging: Methylene blue for skin supports mitochondrial function, reduces oxidative stress, stimulates collagen production, and promotes cellular longevity, contributing to healthier, more resilient skin and slower aging.

• Balances Mood and Relieves Stress: Methylene blue's ability to reduce anxiety and depression symptoms is due to its support of mitochondrial energy production and modulation of neurotransmitters.

• Supports Mental Well-Being and Emotional Health: Methylene blue optimizes mitochondrial energy, regulates neurotransmitters, reduces oxidative stress, and improves cognitive and emotional health.

• Improves Oxygen Flow and Speeds Up Recovery: Methylene blue's benefits in improving oxygen flow and speeding up recovery are driven by its role in improving mitochondrial energy production, reducing oxidative stress, and supporting efficient tissue repair and cellular resilience.

• Aids in Detoxification and Eliminates Toxins: Methylene blue nourishes the body by restoring hemoglobin function, modulating nitric oxide pathways, and removing oxidative stress-related toxins from the bloodstream.

• Combats Inflammation and Fights Infections: Combats inflammation and fights infections, which refers to methylene blue’s ability to regulate immune responses, reduce oxidative stress, and disrupt pathogen activity while preserving immune and tissue function.

• Enhances Sleep Quality: Methylene blue improves sleep quality by stabilizing brain function, reducing oxidative stress, and supporting balanced neurological and circadian activity.

• Strengthens Vision and Protects Eye Health: Methylene blue maintains retinal integrity, reduces oxidative stress, and supports mitochondrial function, preventing visual deterioration and ensuring long-term ocular health.

• Improves Metabolic Efficiency: Methylene blue boosts mitochondrial respiration, produces ATP, and supports glucose metabolism, resulting in sustained energy and reduced physiological stress.

Methylene blue supports mitochondrial function, improves cellular energy production, and reduces oxidative stress, contributing to better brain health, skin vitality, and metabolic efficiency. The antioxidant promotes detoxification by restoring blood oxygen levels and reducing inflammation and infections. Methylene blue promotes mental well-being, stabilizes mood, and supports eye health by protecting retinal cells. Diverse applications include improving sleep quality, accelerating recovery, and potentially supporting longevity through cellular repair and reduced damage.

Enhancing cognitive function and mental clarity is a recognized benefit of methylene blue. Neuroprotection is a key advantage of methylene blue, attributed to its ability to support mitochondrial efficiency and reduce oxidative stress. The compound acts as an electron donor and acceptor within the mitochondrial electron transport chain, ensuring stable ATP production and minimizing the accumulation of reactive oxygen species. The mechanism contributes to sustained neuronal health, improved cognitive function, and better memory retention.

Methylene blue brain benefits have demonstrated potential in improving cognitive function and reducing oxidative stress in a D-galactose-induced Alzheimer's disease mouse model. Research indicates that it promotes learning and memory while lowering amyloid-β levels and oxidative damage in the hippocampus. Methylene blue has been suggested as a therapeutic agent for neurodegenerative conditions, according to an article published in Springer Naturelink on March 26, 2024.

Protecting brain health and preventing degeneration is a benefit of methylene blue linked to its role in mitochondrial support and neuroprotection. Neuroprotection is achieved through methylene blue’s ability to improve mitochondrial respiration, ensuring stable ATP production and reducing oxidative stress in neurons. The mechanism safeguards brain cells from metabolic failure and inflammation, which contribute to neurodegenerative diseases such as Alzheimer’s and Parkinson’s.

The research article examines the potential cognitive benefits of methylene blue, highlighting its role in increasing mitochondrial function and mitigating oxidative stress in the brain. Methylene blue increases ATP production and lowers reactive oxygen species, contributing to memory consolidation and protecting neurons in Alzheimer's disease. Dosing and purity are critical factors in determining its effectiveness and safety, although preliminary findings are promising, according to Healthspan's article. The research, entitled "Methylene Blue for the Aging Brain: Mitochondrial Mechanisms Driving Neuroprotective and Cognitive Benefits," was conducted by Shriya Bakhshi and Daniel Tawfik.

Improved skin health and slowed aging represent benefits of methylene blue. The compound supports mitochondrial function by acting as an efficient redox agent, which reduces oxidative stress in skin cells, contributing to age-related changes such as wrinkles, fine lines, and uneven tone. Methylene blue boosts energy production at the cellular level, maintaining structural and functional integrity in skin cells. Laboratory studies demonstrated that methylene blue increases the proliferation of skin fibroblasts, the cells responsible for producing collagen and other components of the skin’s extracellular matrix. Methylene blue brain benefits have been studied for influencing memory, focus, and cognitive performance, showing improved mitochondrial activity and reduced oxidative damage in neural tissue.

Methylene blue promoted cellular longevity and healthier aging by delaying senescence, a condition in which cells lose their ability to divide and repair tissue. The result was increased skin hydration and a thicker dermal layer, contributing to a more resilient and youthful appearance. The findings indicate that methylene blue for skin supports long-term skin vitality by preserving cellular activity and improving the biological environment of the skin, which leads to longevity and healthier aging through sustained tissue renewal and reduced cellular damage.

Balancing mood and relieving stress represent benefits of methylene blue, attributed to its impact on mitochondrial function and neurotransmitter modulation. Increasing mitochondrial respiration allows methylene blue to increase cellular energy production, which is needed for optimal brain function. The treatment helps stabilize mood and reduce stress levels. Research indicates that methylene blue exhibits antidepressant and anxiolytic effects without inducing mania, making it a potential treatment for mood disorders such as bipolar disorder.

Methylene blue's role in facilitating extinction learning has been explored in the context of post-traumatic stress disorder (PTSD). Stimulating mitochondrial metabolism improves the activity of neurons involved in extinction learning, reducing fear and anxiety responses. The findings suggest that methylene blue's multifaceted mechanisms contribute to its capacity to balance mood and alleviate stress.

Methylene blue supports mental well-being and emotional health by promoting neurological balance, stabilizing mood, and reducing cognitive stress. Mitochondrial function ensures efficient energy production in brain cells, maintaining neural pathways smoothly, and maintaining emotional stability. Methylene blue protects neurons from degeneration by neutralizing oxidative stress, preserving cognitive function, and preventing mood disturbances. Regulating neurotransmitter activity helps maintain a balanced mind, enabling focus, relaxation, and the development of emotional resilience.

Methylene blue strengthens psychological stability by optimizing oxygen utilization, ensuring consistent neural activity without excessive fatigue or mental strain. Stress regulation is facilitated by reducing cellular inflammation, preventing disruptions to cognitive processing, and emotional responses. Supporting neurotransmitter efficiency promotes cognitive flexibility and emotional adaptability, allowing the brain to function at peak performance. The effects contribute to sustained mental clarity, preserving neurological health, and reinforcing emotional strength.

Improving oxygen flow and speeding up recovery refers to methylene blue's ability to support efficient oxygen transport and cellular repair. Methylene blue acts as an electron carrier in the mitochondrial electron transport chain, ensuring optimal energy production, allowing tissues to receive the oxygen needed for cellular regeneration. Stabilizing oxidative balance reduces metabolic stress, preventing cellular damage and accelerating recovery. Increased oxygen utilization increases circulation, supporting vital functions such as muscle repair, tissue healing, and neurological restoration.

Methylene blue strengthens physiological resilience by stabilizing mitochondrial activity, ensuring that energy-dependent processes remain functional under stress conditions. It reduces inflammation and preserves cellular structure by preventing the accumulation of excess free radicals, promoting faster recovery from fatigue and physical strain. Optimal oxygen absorption supports endurance, maintaining sustained energy levels without compromising metabolic integrity. The effects contribute to cellular health, ensuring that physiological systems remain efficient and capable of continuous restoration.

Methylene blue combats inflammation and fights infections by regulating immune responses and eliminating harmful pathogens. The antioxidant properties neutralize oxidative stress, reducing cellular damage and preserving tissue integrity. Excessive oxidative stress contributes to chronic inflammation, impairing normal immune function and increasing susceptibility to infections. Methylene blue helps prevent inflammatory disorders while supporting immune efficiency by maintaining the balance between oxidation and reduction within cells. Protecting cellular structures and preventing immune-related stress promotes faster tissue repair.

Methylene blue strengthens immune defenses by ensuring optimal energy production in immune cells, allowing them to function effectively against bacterial and viral threats. Stabilizing mitochondrial respiration improves cellular resilience, guaranteeing rapid and targeted immune responses. Antimicrobial action disrupts microbial metabolism and weakens pathogens, preventing them from spreading throughout the body. Regulating immune activation contributes to systemic protection, reducing infection-related complications, and maintaining long-term immune health. Maintaining stable inflammatory responses ensures physiological balance, preventing excessive immune reactions and promoting recovery from illness.

Enhancing sleep quality refers to the ability of methylene blue to support restorative sleep by stabilizing brain activity and reducing oxidative stress. The interaction with mitochondrial function ensures efficient energy production, allowing neural processes to operate smoothly during sleep cycles. The stabilization promotes deep sleep and minimizes disruptions, improving mental clarity upon waking. Free electrons are neutralized by methylene blue, which reduces inflammation in the body and promotes relaxation and a balanced neurological state.

Methylene blue aids sleep regulation by influencing neurotransmitter activity and modulating stress responses. Optimizing oxygen utilization helps maintain stable brain function, preventing excessive fatigue or cognitive strain. Circadian rhythm stability fosters a consistent sleep pattern, ensuring sustained rest without unnecessary interruptions. These effects contribute to neurological well-being, allowing the body to recover and maintain physiological balance during sleep.

Strengthens vision and protects eye health, as methylene blue supports retinal function, reduces oxidative stress, and safeguards visual integrity. The antioxidant properties of methylene blue neutralize free radicals that contribute to retinal degeneration, preserving cellular health and preventing damage to the optic nerve. Methylene blue stabilizes mitochondrial activity, ensuring retinal cells' efficient energy production and resilience to physiological stress.

Recent studies highlight methylene blue’s potential in preventing retinal damage and vision deterioration. Research on perinatal asphyxia-induced retinal degeneration demonstrates its ability to regulate inflammation, matrix remodeling, and angiogenesis, reducing neurotoxicity and preserving retinal structure. Investigations into glaucoma suggest that methylene blue mitigates oxidative stress and supports mitochondrial function, protecting retinal ganglion cells from progressive damage. The findings support the therapeutic relevance in maintaining eye health and preventing vision loss, according to an article published in Frontiers on June 4, 2020, by Juan Carlos Fernandez and colleagues.

Improving metabolic efficiency refers to the ability of cells to optimize energy production and utilization, reducing metabolic stress and improving physiological function. Methylene blue facilitates the process as an alternative electron carrier in the mitochondrial electron transport chain, ensuring efficient ATP synthesis. Stabilizing oxidative balance and supporting mitochondrial respiration promote sustained energy output and cellular resilience. Methylene blue’s role in stimulating glucose metabolism increases metabolic efficiency, allowing cells to function optimally under varying physiological conditions.

Recent research has highlighted the impact of methylene blue on metabolic health, demonstrating its ability to increase resting metabolism by up to 42%. Studies indicate that methylene blue improves oxygen consumption rates and ATP production, even in low-oxygen environments, by increasing the activity of mitochondrial complexes I–III. The mechanism supports fat oxidation, energy balance, and cellular recovery, making methylene blue a promising compound for metabolic optimization. Detailed insights into effects on glucose absorption and mitochondrial restoration reinforce the potential to improve metabolic efficiency, according to an article published in ImpactHealth+ in 2025.

Methylene Blue supports bodily processes by improving mitochondrial function, boosting cellular energy production, and reducing oxidative stress. Methylene blue affects the body by interacting directly with mitochondrial function and cellular respiration, acting as an electron donor that bypasses damaged electron transport chains and improves adenosine triphosphate (ATP) production. The process supports energy metabolism in cells under stress, particularly in the brain and muscles, where energy demand is high. Methylene blue reduces oxidative stress by accepting and donating electrons, stabilizing mitochondrial activity, and preventing excess free radical formation.

The antioxidant improves redox balance, promotes oxygen utilization, and protects mitochondria from dysfunction. Low doses of Methylene blue influence neurological activity by increasing the function of cytochrome c oxidase, a crucial enzyme involved in energy generation. These biochemical effects contribute to benefits such as improved cognitive function, neuroprotection, and support for conditions involving cellular stress or metabolic decline.

Methylene blue is considered safe when used appropriately, and safety depends on dosage, individual health conditions, and probable drug interactions. Methylene blue is FDA-approved for treating methemoglobinemia and has neuroprotection and antimicrobial therapy applications. Pregnant women, taking serotonergic medications, and patients with G6PD deficiency must avoid it because of the risk of hemolysis, serotonin syndrome, and fetal exposure. Side effects include nausea, dizziness, and temporary skin or urine discoloration. Consulting a healthcare professional before use ensures proper administration and minimizes adverse effects.

The contents of methylene blue that benefit the body include methylthioninium chloride, a redox-active compound that supports mitochondrial function, antioxidant properties that reduce oxidative stress, and antimicrobial action that helps combat infections. The synthetic process begins with aniline, which undergoes methylation to form N, N-dimethylaniline. The compound is then oxidized using sodium nitrate and sulfuric acid, producing the leuco base of methylene blue. An additional oxidation stabilizes the dye, followed by filtration, precipitation, and recrystallization to ensure purity.

The contents of Methylene Blue that benefit our body are listed below.

• Methylthioninium Chloride (C₁₆H₁₈ClN₃S): Methylthioninium chloride is the primary compound in methylene blue, consisting of carbon, hydrogen, nitrogen, chlorine, and sulfur. The synthetic molecule interacts with biological systems by stabilizing redox reactions and supporting cellular energy production. The ability to regulate oxidative stress contributes to neuroprotection and improved mitochondrial function.

• Redox-Active Structure (Electron Donor and Acceptor): Methylene blue is a redox-active molecule, alternating between oxidized and reduced states. The characteristic facilitates electron transfer in the mitochondrial electron transport chain, improving cellular respiration. Efficient electron flow supports ATP production, reducing metabolic dysfunction and oxidative damage.

• Antioxidant Properties: Methylene blue exhibits antioxidant activity by neutralizing reactive oxygen species and preventing cellular damage. Methylene blue stabilizes oxidative stress, protects neurons from degeneration, and supports cognitive function, contributing to its potential role in neurodegenerative disease management.

• Thiazine Core (Sulfur-Containing Heterocycle): The thiazine core is a sulfur-containing heterocyclic structure that influences methylene blue’s biochemical interactions. The molecular framework increases its ability to modulate enzymatic activity and regulate oxidative balance. The structural properties contribute to therapeutic applications in metabolic and neurological disorders.

• Blood-Brain Barrier Penetration: Methylene blue crosses the blood-brain barrier, allowing direct interaction with neural tissues. The capability influences neurotransmitter regulation and mitochondrial function within the brain. The neuroprotective effects support cognitive health and aid in oxidative stress and neuroinflammation conditions.

• Photosensitizing Properties (Light Absorption): Methylene blue absorbs specific wavelengths of light, activating photodynamic reactions. The property enables its use in antimicrobial therapies, where light exposure strengthens its ability to disrupt pathogenic cells. The photosensitizing effects contribute to applications in targeted medical treatments.

• Antimicrobial and Antiviral Action: Methylene blue exhibits antimicrobial and antiviral properties by interfering with microbial metabolism and replication. Generating oxidative stress disrupts bacterial and viral structures, reducing infection risks. The mechanism supports its use in treating various infectious diseases.

No, methylene blue cannot treat cancer cells. Methylene blue is not approved for cancer treatment, but research has shown that it affects cancer cells under certain experimental conditions. Methylene blue generates reactive oxygen species under light activation, damaging cancer cell structures through photodynamic therapy. The approach has been investigated for its ability to impair mitochondrial respiration and induce oxidative stress in tumor cells without harming healthy tissue.

Research exploring methylene blue and cancer has focused on its role as a photosensitizer in targeted therapies. Using methylene blue for cancer in controlled environments has reduced cell viability in models of breast, prostate, and skin cancers. A regulatory body has not approved methylene blue cancer treatment for clinical use, and current data remain limited to preclinical studies. Studies and trials are needed to determine its safety, effectiveness, and long-term impact on human cancer progression. Methylene blue is not an FDA approved cancer treatment. 

You can get Methylene Blue at licensed pharmacies, scientific supply companies, or health-focused retailers that offer pharmaceutical-grade formulations. Methylene Blue is available in oral capsules, liquid solutions, and intravenous preparations, each suited for specific uses like cognitive support or medical treatment. Pharmaceutical-grade products are recommended for internal use, while industrial-grade solutions are restricted to laboratory or external applications due to impurities and toxic additives.

Era Organics offers a highly purified 1% solution for safe supplementation. It offers lab-tested, pharmaceutical-grade available to buy Methylene Blue 1% drops free of standard contaminants such as heavy metals and industrial dyes. The product is suspended in USP-grade water with precise dosing control, making it suitable for users seeking a clean and consistent methylene blue source for research or wellness purposes.

To take Methylene Blue safely and effectively, follow the five steps below under the guidance of a healthcare professional.

1. Determine the Correct Dosage. Follow weight-based dosing guidelines, such as 0.5 to 4 mg/kg for cognitive support or 2 mg/kg IV for medical treatment. Use calibrated tools for measuring liquid or capsule forms to maintain accuracy.

2. Choose the Proper Form. Select pharmaceutical-grade methylene blue for internal use, and verify that it contains no industrial additives or contaminants. Liquid and capsule forms are used for oral administration, while intravenous use is reserved for clinical settings.

3. Take on an Empty or Lightly Fed Stomach. Absorption is more consistent when methylene blue is taken on an empty stomach. Avoid mixing with acidic beverages, which alter its chemical stability.

4. Avoid Drug Interactions. Do not combine methylene blue with serotonergic medications due to the risk of serotonin syndrome. Check with a healthcare provider if other prescriptions are being taken.

5. Monitor for Side Effects. Watch for signs such as dizziness, nausea, or changes in urine color. Discontinue use and seek medical advice if any symptoms develop.

Methylene blue supplement begins to work within 5 to 30 minutes when administered as an IV infusion, with effects noticeable after a single dose. Results differ depending on whether the drug is used off-label for cognitive development or other applications, with users reporting improvement within a few days to a week. The timeline depends on dosage, metabolism, and the condition being treated.

The recommended dosage of Methylene Blue is between 0.5 and 4 mg/kg for cognitive support and up to 2 mg/kg intravenously for medical treatment such as methemoglobinemia. Dosage varies by purpose, route of administration, individual health condition, and potential drug interactions, requiring professional supervision to ensure safety and therapeutic effectiveness.

The recommended Methylene Blue dosages under the guidance of a healthcare professional are listed below.

• 0.5 to 4 mg/kg oral for Cognitive Support: Methylene blue is used in low doses to support mitochondrial function and improve memory retention. Doses between 0.5 and 4 mg per kilogram of body weight have demonstrated benefits in brain energy metabolism and synaptic activity. The range supports safe use while minimizing the risk of toxicity or oxidative imbalance and has been tested for neuroprotective properties in studies on aging and cognitive decline. A proper methylene blue dosage depends on the intended outcome, formulation, and individual health condition.

• 1 to 2 mg/kg via IV for Methemoglobinemia Treatment : The dosage treats methemoglobinemia, a condition in which hemoglobin loses its ability to carry oxygen. Intravenous administration of 1 to 2 mg per kilogram restores regular oxygen transport by reducing methemoglobin to hemoglobin. Treatment is short-term and closely monitored due to the risk of cardiovascular or hematologic side effects. The therapeutic dosage is supported by FDA approval and clinical protocols.

• 1 to 4 mg/kg through oral or IV for Antimicrobial or Antiviral Use: Methylene blue has been evaluated as an antimicrobial and antiviral agent in controlled settings. A 1 to 4 mg dosage per kilogram supports redox reactions that interfere with microbial activity and replication. The usage remains experimental for most infections, though it has shown promise in combination therapies. Recommended Methylene Blue dosages refer to the safe and effective amounts used for specific therapeutic or nootropic purposes. Medical guidance is needed to ensure safe application and prevent interactions.

• 15 to 60 mg/day oral for Depression or Mood Disorders: Methylene blue is studied in psychiatric applications for its monoamine oxidase-inhibiting properties. Daily doses between 15 and 60 mg have been tested in trials addressing treatment-resistant depression. Monitoring is required due to the risk of serotonin syndrome when combined with serotonergic drugs. Dosage adjustments depend on the patient's medication history and clinical response.

No, it is not safe to take Methylene Blue daily without medical oversight. The safety of daily use depends on dosage, personal health status, and possible drug interactions. Methylene blue has been studied at low doses for probable cognitive and mitochondrial benefits, but extended daily use increases the risk of adverse effects. Serotonin syndrome occurs when combined with antidepressants, hemolytic anemia in individuals with glucose-6-phosphate dehydrogenase (G6PD) deficiency, and disturbances in cardiovascular function when administered intravenously.

The strong oxidative activity interferes with the cell's redox balance, disrupting normal physiological processes if used excessively or without proper monitoring. Evidence from clinical settings supports its short-term use in specific treatments, such as methemoglobinemia or certain infections, but long-term safety data for daily supplementation remain limited. Medical supervision is necessary to assess risks, manage dosage, and prevent complications linked to prolonged exposure.

Yes, there are possible effects of taking Methylene Blue. The drug causes headaches, nausea, dizziness, and blue discoloration of urine or skin due to its chemical properties and metabolic activity. A higher dose of methylene blue is associated with serious side effects, such as serotonin syndrome, when combined with serotonergic medications such as Selective Serotonin Reuptake Inhibitors. The condition involves symptoms such as agitation, confusion, rapid heart rate, and high blood pressure due to excessive serotonin levels in the brain.

Hemolytic anemia occurs when glucose-6-phosphate dehydrogenase (G6PD) deficiency causes the premature breakdown of red blood cells due to oxidative stress. The Methylene Blue side effects illustrate its impact on cellular functions and interactions with the body, demonstrating its profound effect on cellular functioning. Intravenous administration leads to chest pain or abnormal heart rhythms in sensitive individuals. The compound’s strong oxidizing potential explains the adverse effects, as it disrupts normal cellular function in certain physiological conditions. Careful dosing and medical supervision are necessary to avoid these complications.

The possible side effects of taking Methylene Blue are listed below.

• Pain in the arms or legs

• Blue or green discoloration of urine

• Altered sense of taste

• Headache and dizziness

• Muscle or joint pain

• Loss of appetite and general discomfort

• Insomnia and trouble sleeping

• Agitation and restlessness

• Rapid heart rate and chest tightness

• Difficulty breathing or swallowing

• Shivering and tremors

• Confusion and poor coordination

• Allergic reactions, including hives and swelling

People who should not take Methylene Blue are listed below.

• People with Glucose-6-Phosphate Dehydrogenase (G6PD) Deficiency: Individuals with G6PD deficiency risk hemolytic anemia when exposed to methylene blue. The condition destroys red blood cells, resulting in symptoms like fatigue, jaundice, and dark-colored urine.

• Individuals Taking Serotonergic Medications: Patients on serotonergic drugs, such as selective serotonin reuptake inhibitors (SSRIs), monoamine oxidase inhibitors (MAOIs), or certain opioids, must not use methylene blue. The combination increases serotonin levels excessively, leading to serotonin syndrome, a potentially life-threatening condition characterized by symptoms like agitation, confusion, rapid heart rate, and high blood pressure.

• People with Known Hypersensitivity to Methylene Blue: Patients who have previously experienced allergic reactions or anaphylaxis to methylene blue or similar compounds must avoid its use. Re-exposure leads to severe allergic responses, including difficulty breathing, swelling, and hives.

• Pregnant Women: Methylene blue is contraindicated during pregnancy due to potential risks to the fetus. Usage has been associated with fetal harm, and safer alternatives must be considered for treating conditions during pregnancy.

Methylene blue is dangerous for pregnant and breastfeeding mothers because it has been linked to fetal toxicity and potential neonatal harm. The use of methylene blue during pregnancy is associated with serious adverse outcomes, including fetal hemolytic anemia, hyperbilirubinemia, and intestinal atresia during the second trimester or later. The ability to interfere with fetal oxygen transport due to methemoglobinemia represents a direct risk to developing organs and systems. Evidence from case reports and clinical observations indicates that intra-amniotic exposure has caused congenital abnormalities and gastrointestinal complications in newborns.

Methylene blue is excreted into breast milk during lactation, raising concerns about its toxicity for nursing infants. The compound’s oxidative effects disrupt red blood cell function in neonates with glucose-6-phosphate dehydrogenase (G6PD) deficiency, increasing the risk of hemolysis. Medical guidelines recommend avoiding methylene blue during pregnancy and breastfeeding unless no safer alternatives exist and the benefits outweigh the potential harm. The precaution is based on documented physiological vulnerabilities in fetuses and newborns and the drug’s known pharmacological activity.